Intended for advanced readers, this is a review of all relevant techniques for structure analysis. It provides the latest knowledge on spectroscopic and related techniques for chemical structure analysis, such as NMR, optical spectroscopy, mass spectrometry and X-ray crystallography, including the scope and limitation of each method. As a result, readers not only become acquainted with the techniques, but also the advantages of the synergy between them. This enables them to choose the correct analytical method for each problem, saving both time and resources. Special emphasis is placed on NMR and its application to absolute configuration determination and the analysis of molecular interactions. The authors guarantee both solid methodology and applications essential for structure determination, equipping experts as well as newcomers with the tools to solve any structural problem.