OPTIMASI KONDISI OPERASI SINTESIS FexOy NANOPARTIKEL DENGAN CRUDE EXTRACELLULAR METABOLITE Streptomyces cavourensis

Abstract

This study investigates the extracellular synthesis of FexOy nanoparticles using Streptomyces cavourensis (SV-21) crude extracellular metabolite (supernatant), focusing on the optimization of reaction time, FeCl₃ concentration, and temperature. The aim is to determine the effects of these conditions on the morphology, size, and yield of the nanoparticles, as well as to evaluate their biological activity under optimal conditions. The methodology involves the extracellular synthesis of FexOy nanoparticles using Streptomyces sp. extract with variations in temperature, FeCl₃ concentration, and reaction time. Characterization was performed to analyze the morphology, size, and biological activity of the nanoparticles in order to determine the most optimal synthesis conditions. The results show that the optimal synthesis conditions were achieved at a FeCl₃ concentration of 0.05 M, a temperature of 23°C, and a reaction time of 4 hours. SEM characterization revealed particle sizes ranging from 10–60 nm with average size of 29 nm, while SEM, TEM, FTIR, XRD, and EDX analyses confirmed the formation of Fe₃O₄/-Fe2O3. Antibacterial tests showed effectiveness against Staphylococcus aureus but not against E. coli. Further research is recommended using more detailed variations in reaction time and optimization of supernatant volume to prevent agglomeration and enhance antibacterial effectiveness against Gram-negative bacteria.