EKSTRAKSI SENYAWA BIOAKTIF DARI KULIT KAYU MASOYI (Cryptocarya massoia (Oken) Kosterm) MENGGUNAKAN METODE UAE (ULTRASOUND-ASSISTED EXTRACTION) DENGAN PELARUT BERBASIS DES (DEEP EUTECTIC SOLVENTS) SERTA STUDI AKTIVITAS ANTIOKSIDAN

Abstract

Massoia bark (Cryptocarya massoia (Oken) Kosterm.), particularly the bark portion, is known to contain bioactive compounds with potential antioxidant activity. This study aimed to develop an environmentally friendly extraction method (green extraction) using Deep Eutectic Solvents (DES) composed of choline chloride and glycerol, assisted by Ultrasound-Assisted Extraction (UAE). DES were synthesized at molar ratios of 1:2 and 1:3 with concentration variations of 30%, 50%, and 70%, and subsequently applied for the extraction of bioactive compounds at 50°C for 30 minutes. The resulting extracts were analyzed for total phenolic content, total flavonoid content, and antioxidant activity using an ELISA Reader and LC-HRMS. The results demonstrated that variations in DES molar ratio and concentration significantly affected total phenolic content (0.0084 – 0.0321 mg gallic acid/g extract), total flavonoid content (0.0009 – 0.0047 mg quercetin/g extract), and antioxidant activity (20 – 53%). The DES system with a molar ratio of 1:2 at a concentration of 70% exhibited the highest extraction performance. In contrast, increasing glycerol proportion and water content in the DES system led to a decrease in extraction efficiency and antioxidant inhibition percentage. ANOVA analysis confirmed that both DES molar ratio and DES concentration had a significant effect on the antioxidant activity of massoia bark extract.