Ekstraksi Kolagen Dari Kulit Ikan selar (Selaroides Leptolepis) Dengan Metode Larut Asam Dan Penambahan Enzim Pepsin

Abstract

Selar fish (Selaroides Leptolepis) is a type of fish that is widely distributed in Indonesian waters and has the potential to be a source of collagen. This study was conducted to determine the characteristics of acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from the skin of selar fish (Selaroides leptolepis). Collagen was extracted by using the skin of selar fish using the acid solution method and the addition of the enzyme pepsin. The pretreatment process was carried out using 0.1 M NaOH for 18 hours. Selar fish skin samples were then extracted using 0.5 M acetic acid (CH3COOH) for 12 hours and 24 hours, as well as the addition of pepsin enzymes with variations in the concentrations of 0%, 1% and 5%. The results of the collagen obtained were then analyzed for moisture content and ash content, FTIR (Fourier Transform Infrared Spectrophotometry), uv-vis spectrophotometer analysis and DSC (Differential Scanning Calorimetry) analysis. From the extraction results, the increase in collagen yield is directly proportional to the enzyme concentration and extraction time. Optimum conditions were achieved in samples with 5% enzyme concentration with extraction time of 24 hours with a value 18.64 % by mass. The results of the water Content and collagen ash Content were 2.37% and 15.87%. From the FTIR results for 6 sample variations, it shows that the extraction process with acid and pepsin can maintain the triple helix structure of collagen. Based on DSC (Differential Scanning Calorimetry) analysis, the denaturation temperature of pepsin-soluble collagen was 64.05°C.